sos1 overexpression  (Addgene inc)

Journal: Translational Oncology

Article Title: SOS1 promotes epithelial-mesenchymal transition of Epithelial Ovarian Cancer(EOC) cells through AKT independent NF-κB signaling pathway

doi: 10.1016/j.tranon.2021.101160

Figure Lengend Snippet: SOS1 expression in epithelial ovarian cancer (EOC). (A) SOS1 expression in different types of EOC tissue. The SOS1 expression of normal, benign, and carcinoma tissue. Ca: carcinoma, Ben: benign, Nor:normal. (B) Each bar in the histogram represents the mean ± SEM of relative density (*p < 0.05). (C-D) The SOS1 expression in five ovarian cancer cell lines. The histogram indicates the expression intensity of SOS1. Four separate experimental replicates were performed.

Article Snippet: The specific adenovirus vectors overexpression SOS1 and the Negative non-targeting control adenovirus were constructed by Shanghai GeneChem Co. Ltd. (Shanghai, China).

Techniques: Expressing

Journal: Translational Oncology

Article Title: SOS1 promotes epithelial-mesenchymal transition of Epithelial Ovarian Cancer(EOC) cells through AKT independent NF-κB signaling pathway

doi: 10.1016/j.tranon.2021.101160

Figure Lengend Snippet: In vitro experiments demonstrate the involvement of SOS1 in the metastasis of EOC. the Significantly decreased cell migration (A, upper panels), invasion through Matrigel (A, lower panels), intravasation through the basement membrane (Matrigel) and HUVECs (B), and extravasation through HUVECs and Matrigel (C) were observed in Hey cells with siRNA-mediated knockdown of SOS1 (SOS1i cells) in contrast with migration, invasion, intravasation, and extravasation of the negative controls (NT.cont). The controls bar of the histogram (right lanes) represents the mean ± SEM of three experiments, and the schematics for intravasation, and extravasation assays are given (*p < 0.05, **p < 0.01) . In the intravasation and extravasation assay, tumor cells were labeled with Cell Tracker GFP to differentiate extravasated HEY cells from HUVEC cells. Scale bars: A, B 100 μm; C 50 μm.

Article Snippet: The specific adenovirus vectors overexpression SOS1 and the Negative non-targeting control adenovirus were constructed by Shanghai GeneChem Co. Ltd. (Shanghai, China).

Techniques: In Vitro, Migration, Membrane, Knockdown, Labeling

Journal: Translational Oncology

Article Title: SOS1 promotes epithelial-mesenchymal transition of Epithelial Ovarian Cancer(EOC) cells through AKT independent NF-κB signaling pathway

doi: 10.1016/j.tranon.2021.101160

Figure Lengend Snippet: The effect of SOS1 on the EMT of Hey cells. (A) Hey cells cellular morphology, skeleton and cellular pseudopods were observed (*400); (B)The mRNA expression of SOS1, N-cadherin, Vimentin, MMP9, MMP2 and Snail are reduced in Hey cells, but the mRNA expression of E-cadherin is increased. Each bar in the histogram represents the mean ± SEM of relative OD values (*p < 0.05); (C) A similar trend of the protein expression was detected. Each bar in the histogram represents the mean ± SEM of relative OD values (*p < 0.05). Four separate experimental replicates were performed. Scale bars: A 100 μm.

Article Snippet: The specific adenovirus vectors overexpression SOS1 and the Negative non-targeting control adenovirus were constructed by Shanghai GeneChem Co. Ltd. (Shanghai, China).

Techniques: Expressing

Journal: Translational Oncology

Article Title: SOS1 promotes epithelial-mesenchymal transition of Epithelial Ovarian Cancer(EOC) cells through AKT independent NF-κB signaling pathway

doi: 10.1016/j.tranon.2021.101160

Figure Lengend Snippet: Knockdown of SOS1 inhibits the activation of NF-κB pathway. (A-B) Rac-GTP and Ras-GTP were detected in SOS1i cells by using a GST-pull down assay. A reduced Ras-GTP level was detected in SOS1i cells, but not detected in the Rac-GTP level. (C-D) Knockdown of SOS1 suppresses Ras-ERK/P38MAPK/JNK pathway. (E-F) Knockdown of SOS1 inhibits NF-κB pathway. Each bar represents the mean ± SEM of relative OD values (*p < 0.05). Four separate experimental replicates were performed.

Article Snippet: The specific adenovirus vectors overexpression SOS1 and the Negative non-targeting control adenovirus were constructed by Shanghai GeneChem Co. Ltd. (Shanghai, China).

Techniques: Knockdown, Activation Assay, Pull Down Assay

Journal: Translational Oncology

Article Title: SOS1 promotes epithelial-mesenchymal transition of Epithelial Ovarian Cancer(EOC) cells through AKT independent NF-κB signaling pathway

doi: 10.1016/j.tranon.2021.101160

Figure Lengend Snippet: SOS1 increases Snail expression and induces EMT through AKT-independent NF-κB activation. (A) Overexpression of SOS1 promotes migration and invasion of OVCAR3 cells. (B) Overexpression of SOS1 induces the mRNA level of N-cadherin, Vimentin and Snail in OVCAR3 cells, but reduces that of E-cadherin. (C) A similar trend of the protein expression was detected. (D) The inhibitor of p-AKT, MK2206-HCL, inhibits the activation of p-AKT, but could not influence the NF-κB pathway. Each bar in the histogram represents the mean ± SEM of relative OD values (*p < 0.05, **p < 0.01). Four separate experimental replicates were performed. Scale bars: A 50 μm.

Article Snippet: The specific adenovirus vectors overexpression SOS1 and the Negative non-targeting control adenovirus were constructed by Shanghai GeneChem Co. Ltd. (Shanghai, China).

Techniques: Expressing, Activation Assay, Over Expression, Migration

Journal: Translational Oncology

Article Title: SOS1 promotes epithelial-mesenchymal transition of Epithelial Ovarian Cancer(EOC) cells through AKT independent NF-κB signaling pathway

doi: 10.1016/j.tranon.2021.101160

Figure Lengend Snippet: . The metastasis intraperitoneal in nude mice. Hey cells (SOS1i or NT.cont) were injected into the peritoneal cavity of nude mice. Five weeks later, mice were euthanized and analyzed: all mice in the groups had tumors within the peritoneal cavity. (A) Metastatic foci in the abdomen, mesentery and diaphragm are shown. Decreased metastatic lesions were detected within abdomen, especially in the mesentery and diaphragm in the SOS1i group. (B) The number of metastatic lesions in the abdominal cavity of the two groups, according to the target organ, (*p < 0.05, **p < 0.01). (C)SOS1 expression was detected in the implants from the abdomen of mice in the NT.cont and SOS1i groups, (*p < 0.05). Three separate experimental replicates were performed. Scale bars: A 50 mm.

Article Snippet: The specific adenovirus vectors overexpression SOS1 and the Negative non-targeting control adenovirus were constructed by Shanghai GeneChem Co. Ltd. (Shanghai, China).

Techniques: Injection, Expressing